The resource to the url or file is currently unavailable. Paraformaldehyde is recommended for use in the preparation of formalin fixatives for tissues or cells when the samples are to be used in florescence studies. No action shall be taken involving any personal risk or without suitable training. A more efficient and rapid penetrant fixative used in combination with glutaraldehyde and acrolein fixatives.
Show this safety data sheet to the doctor in attendance. They are produced and sold as aqueous solutions of glutaraldehyde, a linear, fivecarbon dialdehyde, containing up to 50 percent active by weight. Paraformaldehyde, 4% in pbs is used to preserve tissues for routine histology. Cdc reproductive health formaldehyde niosh workplace. If your lab is not a regular user of formaldehyde fixatives, there are a couple of easier options that we recommend. Any organic m aterial will inactivate formaldehyde. If swallowed and the victim is conscious, dilute, inactivate, or absorb th e ingested formaldehyde by giving milk, activated charcoal, or water. Paraformaldehyde is used as a fumigant, a disinfectant and in the manufacture of synthetic resins like melamine resin, phenol resin and polyacetal resin. Paraformaldehyde has been used for fixing of cells. No component of this product present at levels greater than or equal to 0.
Electron microscopy sciences 20% paraformaldehyde formaldehyde aqueous solution, 100 ml. Tcl119 please refer disclaimer overleaf product description. Our msds database is an easy to use, do it yourself tool, and best of all. Composition and information on ingredients composition. Paraformaldehyde solution, 4% 4% paraformaldehyde in phosphate buffered saline product code. Limited evidence of carcinogenicity in animal studies iarc. F indicates a substance that is the subject of a section 5f rule under tsca. International inventories component tsca dsl ndsl einecs elincs nlp piccs encs aics china kecl. Paraformaldehyde applications applications the most important use of paraformaldehyde is as a source of formaldehyde groups in the production of many thermosetting resins, together with phenol, urea, melamine, resorcinol and other similar reagents.
Paraformaldehyde stock solution 16% add 8 g of paraformaldehyde to 50 ml of water. Material safety data sheet paraformaldehyde msds section 2. Paraformaldehyde commonly has a slight odor of formaldehyde due to decomposition. Incompatible with brass, steel, copper, acid anhydrides. Page 17 safety data sheet according to 19072006ec, article 31 printing date 19. In case of skin contact wash off with soap and plenty of water. Identification of the substancemixture and of the companyundertaking. Paraformaldehyde is the polymeric form of formaldehyde. Incompatible with brass, steel, copper, acid anhydrides, strong oxidizing agents and strong reducing agents. H334 may cause allergy or asthma symptoms or breathing difficulties if inhaled. Paraformaldehyde forms slowly in aqueous formaldehyde solutions as a. Formaldehyde fixatives the microscopy alliance the. Dow offers a broad line of glutaraldehydebased solutions, which are useful in a wide range of industrial and microbiocidal applications.
Formaldehyde was also present at concentrations of 3. P indicates a commenced pmn substance r indicates a substance that is the subject of a section 6 risk management rule under tsca. Supplied as a 4% paraformaldehyde solution in 1x pbs. Paraformaldehyde revision date 18jan2018 component casno weight % paraformaldehyde 30525894 90 4. Exposure was stopped during the first week for the middle and high dose groups due to. Page 3 of 10 msds formaldehyde difficult, give oxygen. To help prevent exposures, the preparation and use of formaldehyde or paraformaldehyde solutions are to utilize local exhaust ventilation systems such as a fume hood, slot hood, or articulating air exhaust system.
Paraformaldehyde extra pure msds cas 30525894 msds. Store in a cool, dry, wellventilated area away from incompatible substances. Ce is a global chemical and specialty materials company that engineers and manufactures a wide variety of products essential to everyday living. Formaldehyde, the decomposition product of paraformaldehyde, has been listed as a carcinogen by ntp and iarc. It is changed to formaldehyde by heating and by adding small amount of sodium hydroxide. Paraformaldehyde, formadehyde and formalin duke light. All containers of formaldehyde or paraformaldehyde must utilize tight fitting lids to prevent the release of formaldehyde. The standard solution contains 4% paraformaldehyde in 0.
We are happy to announce that we are fully compliant with ghs requirements domestically as well as internationally. Commercially available formaldehyde solutions usually contain 1015% methanol, which prevents the formaldehyde from forming the polymeric paraformaldehyde. Download msds pdf search msds currently available for download bioworld offers formaldehyde 4% in pbs for your research at low price. Your new online msds binder is a place for you to store the material safety data sheets you need to deploy.
Electron microscopy sciences 20% paraformaldehyde formaldehyde aqueous solution, 100 ml 20% paraformaldehyde aq solutn. One option is to purchase methanolfree formaldehyde aq in sealed ampoules. Eye contact rinse immediately with plenty of water, also under the eyelids, for at least 15 minutes. Inflammation of upper respiratory tract tissue was noted in these groups. If possible, do not work with paraformaldehyde in solid form. Thus, a protocol calling for 10% formalin is roughly equivalent to 4% formaldehyde. Paraformaldehyde is a white, crystalline sandlike solid with an odor of formaldehyde. If inhaled if breathed in, move person into fresh air. Beware though, that some solutions have methanol in them to stop polymerization but this could have a negative effect on your sample. Safety data sheet northwest missouri state university. If it is suspected that fumes are still present, the rescuer should wear an appropriate mask or selfcontained breathing apparatus. Is there a big difference between using formaldehyde or.
Methylene oxide, ac4553, ac4554, 41860, 41883 supplier. Exposure was stopped during the first week for the middle and high dose groups due to high mortality. It is used as an agricultural chemical, fungicide, bactericide, analytical reagent and wood preservative. Paraformaldehyde reagentgrade,crystalline 30525894. Firstaid measures general advice if symptoms persist, call a physician. Other companies are charging thousands of dollars to set up accounts and give you access to their msds online database. However i have come across a few methods and i just wanted to know if anyone has any suggestions on which method i should use. Paraformaldehyde solution 4% in pbs cas 30525894 scbt. Formaldehyde, 37% ww safety data sheet according to federal register vol. Limited evidence of carcinogenicity in animal studies. Electron microscopy sciences 157s paraformaldehyde methanol free solution. Paraformaldehyde revision date 18jan2018 polymer made with any freeradical initiator regardless of the amount used. Working with formaldehyde could increase your chances of having fertility problems or miscarriage. Hazard communication program for university of rochester.
Formaldehyde and closely related formalin and glutaraldehyde is an organic chemical usually used in a water. While paraformaldehyde has not been identified as a carcinogen, it should be handled with caution since formaldehyde t he monomer from which it is made is a carcinogen. I need to make up 4% paraformaldehyde to fix some adherent cells. Formaldehyde is a gas which when dissolved in water gives a formaldehyde solution. X listed e indicates a substance that is the subject of a section 5e consent order under tsca. Weigh boats thermometer filter paper funnel 2 beakers wh2o big scooper graduated cylinder ph indicator strips scale hot and stirring plate 1 liter volumetric flask. Formaldehyde occupational safety and health administration. Is there a big difference between using formaldehyde or paraformaldehyde for cell fixation for immunofluorescence. Add 1 n naoh dropwise while stirring until the solution clears. Sigmaaldrich offers a number of paraformaldehyde products. Paraformaldehyde pfa is the smallest polyoxymethylene, the polymerization product of formaldehyde with a typical degree of polymerization of 8100 units. Information on the written hazard communication program for formaldehyde in the workplace and how to readuse the information provided on sdss and labels. If it is necessary to purchase it in solid form, purchase prediluted or preweighed chemical preferably in granular or flake, rather than powder form in the least quantity needed to perform work.
It is also used in the preparation of formalin fixatives for tissues or cells. Here, you can learn more about formaldehyde and related chemicals and what you can do to reduce your exposure for a healthier pregnancy. The medical surveillance program, including a description of the potential health hazards and the signs and symptoms of exposure to formaldehyde. This solution may be stored at room temperature for at least one year without change of the original quality. Paraformaldehyde pfa solutions we make 16% pfa stock solution, and make 4% pfa for animal perfusion and slice fixation. Use precautions against formaldehyde exposure when opening containers or entering a poorly ventilated storage area.
Safety data sheet paraformaldehyde suppliers details. Paraformaldehyde can be quite hazardous to handle and it is often difficult to get it to go into solution. It also serves as a fixative in electron microscopy. Paraformaldehyde is the most commonly used fixative employed for fixation of tissues and cells in immunohistochemistry and immunocytochemistry protocols. Paraformaldehyde 4% aqueous solution, em grade contd. Formaldehyde formaldehyde is a colorless, strongsmelling gas often found in aqueous waterbased solutions. Formaldehyde, 20% vv aqueous solution msds msdsdigital. View product specific information, msds, references and buying faq. Commonly used as a preservative in medical laboratories and mortuaries, formaldehyde is also found in many products such as chemicals, particle board, household products, glues, permanent press fabrics, paper product coatings, fiberboard, and plywood. Paraformaldehyde solution 4% in pbs is commonly used to preserve tissues for routine histology in many labs. Formaldehyde, formalin, paraformaldehyde safe work practices. Other companies are charging thousands of dollars to set up accounts and give you access to their msds. However, we make no warranty of merchantability or any.